ASTM E1428 "Pink Stain Test"

Some materials may become discolored or stained when microorganisms grow on them in the environment. The ASTM E1428 standard is used to evaluate the ability of synthetic polymeric materials containing antimicrobial agents to resist such staining, using the indicator staining organism Streptoverticullium reticulum.

ASTM D3274 Fungal Defacement Rating Method

The ASTM D3274 Method details a standardized rating system designed to evaluate the degree of fungal resistance of surface coatings (e.g. paint films). This method is used for tests such as the ASTM D3273. Ratings are based on the percentage of surface fungal coverage and qualitative descriptions. While this method was originally designed for field observations, it may also be applied to observations made within the laboratory.

ASTM G29 Test for Resistance to Algae

The ASTM G29 test method evaluates an antimicrobial surface's ability to resist algal attack. Such testing is important because algae are ubiquitously found in and around bodies of water. Many of these aqueous environments are associated with human activities, such as swimming pools. Under the right conditions, algae can propagate to form large macrofilms that can pervade these structures and render them unsightly and sometimes hazardous.

ASTM E2315 - Liquid Suspension Time-Kill Test

The liquid suspension time-kill test is excellent for disinfectant product developers because it is a fast, relatively inexpensive, and reproducible way to measure the biocidal potential of a liquid antimicrobial formulation. It consists of directly inoculating a liquid test substance with a high concentration of test microorganisms and then determining the percentage killed over time.

The suspension-based time-kill test has been standardized by ASTM, as ASTM E2315 "Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure."

ASTM E1054 Neutralization Evaluation Test

The method is designed to test neutralizing agents for the purpose of inactivating, or quenching, active antimicrobial agents to ensure no inhibitory effects on microorganisms targeted for recovery. The method offers a wide array of neutralization options for test substances. Detailed within the method are the guidelines for neutralization assays with recoveries based in semi-solid medium, liquid medium, and membrane filtration.

EPA Test Method for Residual Self-Sanitizing Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

The residual self-sanitization (RSS) method, EPA Protocol # 01-1A, is the only method which can currently be used to substantiate long-term sanitization claims with the EPA. It evaluates the residual sanitizing efficacy of antimicrobial products after application to inanimate, nonporous, and non-food contact hard surfaces.

This test method was initially devised by Clorox and submitted to the EPA to support claims for an innovative spray product.